Bleach gel: A simple agarose gel for analyzing RNA quality
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Bleach gel: a simple agarose gel for analyzing RNA quality.
RNA-based applications requiring high-quality, non-degraded RNA are a foundational element of many research studies. As such, it is paramount that the integrity of experimental RNA is validated prior to cDNA synthesis or other downstream applications. In the absence of expensive equipment such as microfluidic electrophoretic devices, and as an alternative to the costly and time-consuming standa...
متن کاملAgarose gel electrophoresis.
MATERIALS 6x Gel-loading buffer Agarose solutions (please see Step 3) DNA samples DNA size standards Samples of DNAs of known size are typically generated by restriction enzyme digestion of a plasmid or bacteriophage DNA of known sequence. Alternatively, they are produced by ligating a monomer DNA fragment of known size into a ladder of polymeric forms. DNA staining solution For a discussion of...
متن کاملAgarose gel electrophoresis.
Agarose gel electrophoresis is used to separate DNA or RNA molecules based upon their size.
متن کاملAlkaline agarose gel electrophoresis.
INTRODUCTION Alkaline agarose gels are run at a pH that is sufficiently high to denature double-stranded DNA. The denatured DNA is maintained in a single-stranded state and migrates through the alkaline gel as a function of its size. Alkaline agarose gels are used chiefly to measure the size of first and second strands of cDNA (Construction of cDNA Libraries Stage 1: Synthesis of First-strand c...
متن کاملAgarose gel separation/isolation of RNA-protein complexes.
In many methods currently used to analyze RNA-protein complexes, high salt or other stringent treatments are required for reducing nonspecific interactions and resolving the complex of interest. RNA-protein complexes often dissociate on native polyacrylamide gels and can only be detected on density gradients or by gel filtration. Agarose gel electrophoresis provides an alternative method that i...
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ژورنال
عنوان ژورنال: ELECTROPHORESIS
سال: 2012
ISSN: 0173-0835,1522-2683
DOI: 10.1002/elps.201100335